• 成都軍區(qū)總醫(yī)院全軍普外中心(成都610083);

目的  探討臨床肝移植過程中離體供肝低溫保存—再灌注損傷與肝細(xì)胞凋亡的關(guān)系。
方法  采用細(xì)胞凋亡原位末端標(biāo)記法并結(jié)合電鏡觀察，檢測(cè)低溫保存時(shí)間分別為0、3、6、9小時(shí)的4組兔肝臟在低溫保存—再灌注過程中肝細(xì)胞凋亡發(fā)生情況。
結(jié)果  在低溫保存后的再灌注期間，4組動(dòng)物的肝組織內(nèi)均可見明顯的肝實(shí)質(zhì)細(xì)胞凋亡現(xiàn)象，而且低溫保存時(shí)間越長(zhǎng)的肝臟，其再灌注后產(chǎn)生的肝實(shí)質(zhì)凋亡細(xì)胞數(shù)量越多； 但各組肝臟于低溫保存末，其組織內(nèi)則未見或僅偶見肝實(shí)質(zhì)細(xì)胞凋亡現(xiàn)象。
結(jié)論  肝實(shí)質(zhì)細(xì)胞凋亡顯著參與了肝臟低溫保存—再灌注損傷過程。

引用本文： 湯禮軍,田伏洲,王雨,李曉軍. 離體肝臟低溫保存—再灌注期間肝細(xì)胞凋亡現(xiàn)象觀察. 中國(guó)普外基礎(chǔ)與臨床雜志, 2000, 7(4): 214-216. doi: 復(fù)制

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2. 湯禮軍， 田伏洲， 王雨等. 兔肝臟保存再灌注損傷模型的建立及評(píng)價(jià) 〔Ｊ〕. 肝膽外科雜志， 1998； 6(2)∶123.
3. Gavrieli Y, Sherman Y, BENSasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation 〔Ｊ〕. J Cell Biol, 1992; 119(1)∶493.
4. Shah KA, Shurey S, Green CJ. Characterization of apoptosis in intestinal ischemia reperfusion injurya light and eletron microscopic study 〔Ｊ〕. Int J Exp Pathol, 1997; 78(5)∶355.
5. GraslKraupp B, RuttkayNedecky B, Koudelka H, et al. In situ detection of fragmented DNA (Tunel assay) fails to discriminate among apoptosis, necrosis and autolytic cell death: a cautionary note 〔Ｊ〕. Hepatology, 1995; 21(5)∶1465.
6. Schumer M, Colombel MC, Sawczuk IS, et al. Morphologic biochemical and molecular evidence of apoptosis during the reperfusion phase after brief periods renal ischemia 〔Ｊ〕. Am J Pathol, 1991; 140(4)∶831.
7. Gantner F, Leist M, Jilg S, et al. Tumor necrosis factorinduced hepatic DNA fragmentation as an early marker of T celldependent liver injury in mice 〔Ｊ〕. Gastroenterology, 1995; 109(1)∶166.
8. 印彤， 童善慶， 謝玉才等. 超抗原SEB和D氨基半乳糖對(duì)小鼠肝細(xì)胞的作用觀察 〔Ｊ〕. 免疫學(xué)雜志， 1998； 14(1)∶36.
  1. 1. Jamieson NV, Lindell S, Sundberg R, et al. An analysis of the components in UW solution using the isolated perfused rabbit liver 〔Ｊ〕. Transplantation, 1988; 46(4)∶512.
  2. 2. 湯禮軍， 田伏洲， 王雨等. 兔肝臟保存再灌注損傷模型的建立及評(píng)價(jià) 〔Ｊ〕. 肝膽外科雜志， 1998； 6(2)∶123.
  3. 3. Gavrieli Y, Sherman Y, BENSasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation 〔Ｊ〕. J Cell Biol, 1992; 119(1)∶493.
  4. 4. Shah KA, Shurey S, Green CJ. Characterization of apoptosis in intestinal ischemia reperfusion injurya light and eletron microscopic study 〔Ｊ〕. Int J Exp Pathol, 1997; 78(5)∶355.
  5. 5. GraslKraupp B, RuttkayNedecky B, Koudelka H, et al. In situ detection of fragmented DNA (Tunel assay) fails to discriminate among apoptosis, necrosis and autolytic cell death: a cautionary note 〔Ｊ〕. Hepatology, 1995; 21(5)∶1465.
  6. 6. Schumer M, Colombel MC, Sawczuk IS, et al. Morphologic biochemical and molecular evidence of apoptosis during the reperfusion phase after brief periods renal ischemia 〔Ｊ〕. Am J Pathol, 1991; 140(4)∶831.
  7. 7. Gantner F, Leist M, Jilg S, et al. Tumor necrosis factorinduced hepatic DNA fragmentation as an early marker of T celldependent liver injury in mice 〔Ｊ〕. Gastroenterology, 1995; 109(1)∶166.
  8. 8. 印彤， 童善慶， 謝玉才等. 超抗原SEB和D氨基半乳糖對(duì)小鼠肝細(xì)胞的作用觀察 〔Ｊ〕. 免疫學(xué)雜志， 1998； 14(1)∶36.