【摘要】 目的 探討佛波酯激活的蛋白激酶C與扭轉(zhuǎn)蛋白A在亞細(xì)胞成分中的表達(dá)之間的關(guān)系?！》椒ā〔捎妹庖邿晒夥ㄓ^察扭轉(zhuǎn)蛋白A在原代培養(yǎng)的神經(jīng)元和小鼠胚胎成纖維細(xì)胞（NIH 3T3細(xì)胞）中的分布。運(yùn)用蛋白質(zhì)印跡法分析蛋白激酶C和扭轉(zhuǎn)蛋白A在細(xì)亞細(xì)胞成分中的表達(dá)?！〗Y(jié)果 扭轉(zhuǎn)蛋白A在NIH 3T3細(xì)胞中的表達(dá)類(lèi)似于神經(jīng)元。扭轉(zhuǎn)蛋白A在細(xì)胞質(zhì)溶質(zhì)、膜成分中均有分布。佛波酯活化蛋白激酶C后并不引起扭轉(zhuǎn)蛋白A在細(xì)胞質(zhì)成分和膜成分中表達(dá)含量的變化?！〗Y(jié)論 扭轉(zhuǎn)蛋白A可能是膜相關(guān)蛋白，細(xì)胞氧化應(yīng)激中扭轉(zhuǎn)蛋白A表達(dá)上調(diào)和重分布變化不是由佛波酯誘導(dǎo)的蛋白激酶C活化途徑來(lái)實(shí)現(xiàn)的。鑒于扭轉(zhuǎn)蛋白A表達(dá)上調(diào)具有潛在的治療原發(fā)性早發(fā)扭轉(zhuǎn)性肌張力障礙的前景，影響其分布和表達(dá)的分子機(jī)制需要進(jìn)一步研究。【Abstract】 Objective To investigate the relationship between the phorbol 12-myristate 13-acetate (PMA) activated protein kinase C (PKC) and the subcellular expression of TorsinA protein. Methods The expression of TorsinA in the primary cultured neurons and the NIH 3T3 cells was detected by immunofluorescence. The expression of PKC and TorsinA in subcellular fraction was analyzed by the western blotting. Results The expression pattern of TorsinA in NIH 3T3 cells was similar to neuron. PMA, an activator of PKC, did not promote the up-expression of TorsinA or redistribution in the subcellular fraction of NIH 3T3 cells. Conclusions TorsinA may be a membrane-associated protein. The up-regulation and redistribution of TorsinA is not caused by the pathway of the PMA activating PKC after cells insulted by oxidative stress. We should pay more attention on the mechanisms of the expression of TorsinA protein for the potential therapies to early-onset primary torsion dystonia (DYT1).