【摘要】 目的 觀察胎羊?qū)m內(nèi)心臟介入手術(shù)胎羊血?dú)饧把獫{炎性細(xì)胞因子的變化。方法 8只懷孕雙胎山羊,雙胎之一為實(shí)驗(yàn)組,在相同麻醉?xiàng)l件下,實(shí)驗(yàn)組進(jìn)行胎羊心臟介入治療,并抽取血樣標(biāo)本。監(jiān)測(cè)胎羊的心率、血?dú)?、乳酸?運(yùn)用ELISA法檢測(cè)治療組及對(duì)照組胎羊白介素(IL)1、IL6、IL8及腫瘤壞死因子(TNFα)。結(jié)果 2只胎羊因手術(shù)中發(fā)生心包填塞死亡,存活的6只胎羊手術(shù)前pH值較手術(shù)后有明顯下降(Plt;005),手術(shù)前后乳酸濃度上升(Plt;005),PCO2、PO2差異無(wú)統(tǒng)計(jì)學(xué)意義(Pgt;005),手術(shù)前血漿IL1、IL6、IL8的濃度較手術(shù)后高(Plt;005),手術(shù)前后TNFα的濃度變化無(wú)統(tǒng)計(jì)學(xué)意義(Pgt;005)。結(jié)論 胎羊?qū)m內(nèi)心臟介入手術(shù)可引起胎羊血漿pH值下降,乳酸濃度上升,及細(xì)胞因子IL1、IL6、IL8濃度上升。【Abstract】 Objective To observe the change of blood gas and inflammatory cytokines during intrauterine cardiac intervention surgery on the fetal lambs. Methods Eight pregnant goats with two fetal in each goat were included. With the same anesthesia condition, one of the twin fetus was chose to perform the intrauterine cardiac intervention surgery. The fetal heart beating rate was monitored, and blood samples of the fetus were taken to do the blood gas analysis and to detect the concentration of inflammatory cytokines (IL1, IL6, IL8, and TNFα). Results Two of the eight fetal lambs which was died in the operation because of pericardial tapenade. In the other six survived fetus, the PH was lower than after the surgery, and the concentrations of lactic acid, IL1, IL6, and IL8 are higher than after the surgery. There was no significant difference of PCO2,PO2 and TNFα between before and after the surgery. Conclusion The intrauterine cardiac intervention surgery can make the PH of fetal plasma lower and the concentrations of lactic acid and IL1, IL6, IL8 higher.