【摘要】 目的 探討微弧氧化（microarc oxidation，MAO）結(jié)合應(yīng)用于純鈦種植體表面處理的可行性。 方法 根據(jù)對(duì)純鈦鈦片處理的不同將實(shí)驗(yàn)分為對(duì)照組（A組，不作處理）、MAO組（B組，純鈦片上進(jìn)行MAO處理）及MAO加Ⅰ型膠原組（C組，純鈦片上MAO處理后吸附Ⅰ型膠原）。將成骨細(xì)胞培養(yǎng)于各組鈦片上，通過掃描電鏡、MTT法檢測不同時(shí)間點(diǎn)各組鈦片表面的細(xì)胞生長及增殖情況，并檢測堿性磷酸酶（alkaline phosphatase，ALP）活性?！〗Y(jié)果 掃描電鏡顯示成骨細(xì)胞在C組鈦片上細(xì)胞黏附情況優(yōu)于A、B組；MTT法及ALP活性檢測示培養(yǎng)3、6 d，成骨細(xì)胞在C組鈦片上的增殖及ALP活性與A、B組比較差異均有統(tǒng)計(jì)學(xué)意義（Plt;0.05）?！〗Y(jié)論 MAO結(jié)合Ⅰ型膠原處理的鈦片可更有效提高成骨細(xì)胞表面附著、增殖，且具有較高的ALP活性?！続bstract】 Objective To study the feasibility of applying microarc oxidation (MAO) with collagen Ⅰ in surface modification of pure titanium. Methods According to different processing methods, the pure titanium was divided into three groups: the control group (without surface modification, group A), MAO group (with microarc oxidation applied in pure titanium surface modification, group B), MAO+Ⅰ group (with microarc oxidation and collagen Ⅰ applied in pure titanium treatment, group C). Osteoblasts were cultured on the surface of titanium in each group, and the cell proliferation in each group was detected at different time points by scanning electron microscopy and MTT method. Moreover, the activity of alkaline phosphatase (ALP) was also detected. Results Scanning electron microscopy showed that adhesion of osteoblasts for group C was better than group A and group B. MTT method and ALP activity detection indicated that there was a significant difference between group C and group A, B in cell proliferation and ALP activity on the third and sixth day of cultivation (Plt;0.05). Conclusion MAO with collagen Ⅰ applied in surface modification of pure titanium may increase osteoblast attachment, and promote its proliferation and ALP activity.