【摘要】 目的 觀察機械通氣對黏蛋白（mucin，MUC）-5AC表達的影響及復方川貝精片的干預作用?！》椒ā⌒挛魈m兔25只，6個月齡，雄性；隨機分為對照組、機械通氣12 h組及復方川貝精片低、中、高劑量組。收集支氣管灌洗液，分別采用實時熒光定量聚合酶鏈式反應法和酶聯(lián)免疫吸附試驗檢測支氣管灌洗液中p38 MAPK mRNA，MUC-5AC蛋白和mRNA的表達。 結(jié)果 機械通氣能增強MUC-5AC的分泌（Plt;0.05）；加用復方川貝精片能降低機械通氣后MUC-5AC蛋白和mRNA的表達（Plt;0.05）；復方川貝精片中、高劑量組與低劑量組比較，能降低機械通氣后MUC-5AC蛋白和mRNA的表達（Plt;0.05）?！〗Y(jié)論 機械通氣能促進支氣管黏膜上皮細胞分泌MUC-5AC，復方川貝精片能抑制機械通氣所致MUC-5AC表達升高，其機制可能與其抑制p38 MAPK表達有關(guān)?！続bstract】 Objective To observe the effect of mechanical ventilation by breathing machine on the expression of mucin (MUC-5AC) and the interfering effect of compound tablet of fritillary bulb. Methods New Zealand Rabbits were randomly divided into control group, twelve-hour mechanical ventilation group, and low, medium and high-dose compound tablet of fritillary bulb group. Contents of p38 MAPK mRNA, MUC-5AC mRNA and protein in bronchial irrigating solution were detected by realtime RT-PCR and ELISA methods. Results Mechanical ventilation could increase the expression of MUC-5AC in bronchial irrigating solution (Plt;0.05). Compound tablet of fritillary bulb could decrease the expression of MUC-5AC mRNA and protein after mechanical ventilation (Plt;0.05). Compared with low-dose compound tablet of fritillary bulb group, the expression of MUC-5AC mRNA and protein was lower for the high and medium-dose groups (Plt;0.05). Conclusions Mechanical ventilation can promote the expression of MUC-5AC in bronchial endothelial cells, which can be suppressed by compound tablet of fritillary bulb. This may be due to the suppression effect of p38 MAPK expression.