目的研究外源性胰島素對(duì)糖尿病大鼠缺血后肢血管內(nèi)皮生長因子(VEGF)表達(dá)的影響及其促血管新生的作用。方法取20只健康雄性SD大鼠,將其右后肢股動(dòng)、靜脈及其分支和屬支結(jié)扎，制成糖尿病大鼠后肢缺血模型，然后將其用簡單隨機(jī)化方法隨機(jī)平均分為模型組與治療組，另取10只正常大鼠作為對(duì)照組。14 d后處死大鼠，應(yīng)用 Western blot法檢測大鼠后肢肌肉組織中VEGF蛋白表達(dá)水平，并采用堿性磷酸酶(AKP)染色法測定大鼠后肢肌肉組織中毛細(xì)血管密度。結(jié)果對(duì)照組大鼠術(shù)前和術(shù)后7 d體重和血糖水平比較差異無統(tǒng)計(jì)學(xué)意義(Pgt;0.05); 模型組大鼠術(shù)后7 d與術(shù)前比較，體重明顯下降(Plt;0.05)，但血糖水平差異無統(tǒng)計(jì)學(xué)意義(Pgt;0.05); 而治療組給予皮下注射胰島素注射液術(shù)后7 d較術(shù)前體重明顯下降，并且血糖水平較術(shù)前也明顯下降，差異均有統(tǒng)計(jì)學(xué)意義(Plt;0.05)。與對(duì)照組比較，治療組及模型組大鼠術(shù)后7 d體重明顯下降、血糖明顯升高(Plt;0.05，Plt;0.01); 治療組與模型組比較，體重差異無統(tǒng)計(jì)學(xué)意義(Pgt;0.05)，但治療組大鼠術(shù)后7 d血糖水平較模型組明顯降低(Plt;0.05)。治療組大鼠缺血肢體肌肉組織中VEGF蛋白相對(duì)表達(dá)量(155.06±10.26)明顯高于模型組(94.30±11.23)，Plt;0.05; 對(duì)照組大鼠未檢測到VEGF蛋白表達(dá)。對(duì)照組大鼠右后肢肌肉組織中毛細(xì)血管密度明顯高于模型組和治療組(Plt;0.05)，而治療組又明顯高于模型組(Plt;0.05)。3組大鼠左后肢毛細(xì)血管密度差異無統(tǒng)計(jì)學(xué)意義(Pgt;0.05); 對(duì)照組大鼠左、右后肢毛細(xì)血管密度差異無統(tǒng)計(jì)學(xué)意義(Pgt;0.05); 模型組和治療組大鼠右后肢毛細(xì)血管密度均明顯低于左后肢(Plt;0.05)。結(jié)論胰島素可以增強(qiáng)糖尿病大鼠后肢缺血肌肉組織中VEGF蛋白表達(dá)，促進(jìn)毛細(xì)血管生成，發(fā)揮保護(hù)作用。
本文研究殼聚糖-左氧氟沙星長效緩釋敷料的皮膚刺激性和燒傷治療效果。通過參照GB/T16886.10-2005評(píng)價(jià)該敷料的皮膚刺激性,同時(shí)制備燒傷兔模型,分為三組,空白對(duì)照組用凡士林油紗包扎,陽性對(duì)照組用納米銀創(chuàng)傷貼包扎,實(shí)驗(yàn)樣品組用殼聚糖-左氧氟沙星長效緩釋敷料包扎,分別于3、6、9、14、21、28 d進(jìn)行傷口未愈合面積測量、羥脯氨酸含量及組織病理學(xué)檢查。實(shí)驗(yàn)結(jié)果顯示該敷料原發(fā)性刺激指數(shù)為0;實(shí)驗(yàn)樣品組和陽性對(duì)照組在6、9、14、21 d時(shí),未愈合傷口面積均小于空白對(duì)照組(P＜0.05),實(shí)驗(yàn)樣品組在9 d和14 d時(shí)其未愈合傷口面積顯著低于陽性對(duì)照組(P＜0.05);實(shí)驗(yàn)樣品組在6、9和14 d時(shí)羥脯氨酸含量顯著高于陽性對(duì)照組和空白對(duì)照組(P＜0.05);通過病理學(xué)觀察,在21 d時(shí)實(shí)驗(yàn)樣品組最早出現(xiàn)附屬器。由此說明殼聚糖-左氧氟沙星長效緩釋敷料的皮膚刺激性極輕微,能夠有效促進(jìn)燒傷創(chuàng)面愈合。
【摘要】 目的 研究2型糖尿?。╰ype 2 diabetes mellitus，T2DM）合并非酒精性脂肪性肝?。╪on-alcoholic fatty liver disease， NAFLD）患者血漿脂肪細(xì)胞特異性脂肪酸結(jié)合蛋白（adipocyte-specific fatty acid-binding protein，A-FABP）的水平及其相關(guān)因素?！》椒ā?009年10月—2010年10月選取T2DM合并NAFLD組（A組）60例，未合并NAFLD組56例（B組）為研究對(duì)象。測定體質(zhì)量指數(shù)（body mass index，BMI），檢測血脂、糖化血紅蛋白（hemoglobin A1c，HbA1c）等生化指標(biāo)。放射免疫法測定空腹胰島素（fasting insulin，F(xiàn)INS），空腹C肽水平（fasting C-peptide，F(xiàn)CP），計(jì)算胰島素抵抗指數(shù)（homeostasis model of assessment-insulin resistance，HOMA-IR）、胰島素敏感指數(shù)（insulin sensitivity index，ISI），測定A-FABP、C反應(yīng)蛋白（C-reaction protein，CRP）及腫瘤壞死因子-α（tumor necrosis factor-α，TNF-α）?！〗Y(jié)果 與B組患者相比，A組患者其血漿A-FABP水平、BMI、腰圍、腰臀比、丙氨酸氨基轉(zhuǎn)移酶、門冬氨酸氨基轉(zhuǎn)移酶、CRP、TNF-α、FCP、FINS、總膽固醇、甘油三酯、Ln（HOMA-IR）升高，Ln（ISI）降低，差異有統(tǒng)計(jì)學(xué)意義（Plt;0.05）；兩組HbA1c差異無統(tǒng)計(jì)學(xué)意義（Pgt;0.05）。A-FABP水平變化與TNF-α、HOMA-IR、CRP呈正相關(guān)，與ISI呈負(fù)相關(guān)?！〗Y(jié)論 T2DM伴NAFLD中，A-FABP升高與胰島素抵抗是并存的，且存在明顯相關(guān)關(guān)系，二者在疾病的發(fā)生發(fā)展中均可能具有重要的作用?！続bstract】 Objective To analyze the serum level of adipocyte-specific fatty acid-binding protein (A-FABP) in patients with type 2 diabetes mellitus (T2DM) complicated with non-alcoholic fatty liver disease (NAFLD), and its related factors. Methods From October 2009 to October 2010, 112 patients with T2DM were categorized into two groups: the group with NAFLD (group A) with 60 patients, and the group without NAFLD (group B) with 56 patients. Body mass index (BMI), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and hemoglobin A1c (HbA1c) were detected. Radioimmunoassay was carried out to measure fasting insulin (FINS) and fasting C-peptide (FCP), and homeostasis model of assessment-insulin resistance (HOMA-IR) and insulin sensitivity index (ISI) were calculated. At the same time, A-FABP, C-reaction protein (CRP) and tumor necrosis factor-α (TNF-α) were also detected. Results Compared with patients in group B, plasma levels of A-FABP, BMI, waistline, waist to hip ratio, ALT, AST, TG, TC, HDL-C, LDL-C, CRP, FCP, FINS, and HOMA-IR for patients in group A were all higher, while ISI was lower; and the differences in the above-mentioned parameters were statistically significant (Plt;0.05). The levels of HbA1c in the two groups were not significantly different (Pgt;0.05). The change of A-FABP level was positively correlated with TNF-α, HOMA-IR and CRP, while it wasnegatively correlated with ISI. Conclusions In patients with T2DM with NAFLD, there is an obvious correlation between the coexisting A-FABP rise and insulin resistance. Both of them played critical roles in the onset and developing of the disease.