目的：了解大鼠腦出血后血腫周圍組織細(xì)胞凋亡與神經(jīng)元特異性烯醇化酶（NSE）的表達(dá)及大鼠神經(jīng)功能缺損程度的關(guān)系。方法：用膠原酶注入到大鼠尾狀核的方法制作腦出血模型。將大鼠分為腦出血、假手術(shù)組、正常組3組。采用蘇木素伊紅(HE) 染色、NSE免疫組織化學(xué)染色及TUNEL分別觀察各組在腦出血后第6 h、12 h、24 h、48 h、72 h、5 d、7 d時(shí)血腫周圍NSE及TUNEL的表達(dá)。用Longa評(píng)分法評(píng)價(jià)大鼠神經(jīng)功能缺損程度。結(jié)果：大鼠在膠原酶注入6 h后形成穩(wěn)定的血腫，在造模24～48 h神經(jīng)功能缺損程度最重；6 h即見到TUNEL陽(yáng)性細(xì)胞的表達(dá)，在48 h最明顯；NSE從神經(jīng)元中漏出彌散到細(xì)胞間隙也在48 h達(dá)高峰。結(jié)論：腦出血血腫周圍凋亡與神經(jīng)功能缺損及NSE的變化有關(guān)，凋亡可能在腦出血的神經(jīng)損傷中起重要的作用。
摘要:目的:分析高膽紅素血癥新生兒血清神經(jīng)元特異性烯醇化酶(NSE)含量和新生兒行為神經(jīng)能力測(cè)評(píng)(Neonatal Behavioral Neurological Assessment,NBNA)的變化,探討高膽紅素血癥新生兒血清NSE含量變化的臨床意義。方法:應(yīng)用放射免疫分析法分別測(cè)定60例高膽紅素血癥新生兒和20例對(duì)照組新生兒血清NSE含量,同步測(cè)定血清總膽紅素(TSB),進(jìn)行NBNA評(píng)分;高膽紅素血癥組早期干預(yù)后再次測(cè)定血清NSE含量。結(jié)果: 與對(duì)照組比較,高膽紅素血癥新生兒血清TSB、NSE含量顯著升高,而NBNA評(píng)分明顯降低,差異有顯著性意義(Plt;0.01);對(duì)照組與高膽紅素血癥新生兒輕度增高、中度增高、重度增高四組兩兩比較(均Plt;0.05),存在顯著性差異;血清NSE含量與NBNA評(píng)分呈明顯負(fù)相關(guān)(r=-0628,Plt;0.01);高膽紅素血癥新生兒經(jīng)早期干預(yù)治療后,血清NSE含量均下降(Plt;0.05),差異有顯著性。結(jié)論: 高膽紅素血癥可導(dǎo)致新生兒腦損傷,血清NSE含量可以作為腦損傷的監(jiān)測(cè)指標(biāo)。Abstract: Objective: To analyze levels of neuronspecific enolase(NSE)in serum and neonatal behavioral neurological assessment (NBNA), to study whether NSE in serum can be used as a tool for the early identification of brain damage in neonatal hyperbilirubinemia. Methods: Serum NSE level of 60 full term infants with hyperbilirubinemia and 20 cases as to control group were measured by radioimmunoassay; Also total serum bilirubin (TSB) and NBNA were detected. In the hyperbilirubinemia group,serum NSE level were measured second when TSB were less than 855 μmol/L(5 mg/dL). Results: Compared with control group,the levels of serum TSB、NSE of the hyperbilirubinemia group were significantly higher, but NBNA score was significantly lower. The levels of serum NSE was significantly negative related to NBNA score. In the hyperbilirubinemia group, serum NSE level were significantly lower after treatment. Conclusion: Hyperbilirubinemia in neonates can cause brain damage. Serum NSE level could work as monitoring indexes of this damage.
目的系統(tǒng)評(píng)價(jià)神經(jīng)元特異性烯醇化酶（NSE）對(duì)惡性胸腔積液的診斷價(jià)值。 方法計(jì)算機(jī)檢索TheCochrane Library（2012年第1期）、EMbase、MEDLINE、CBM、CNKI、WanFang Data和VIP數(shù)據(jù)庫(kù)，查找有關(guān)NSE對(duì)惡性胸腔積液診斷價(jià)值的研究，檢索時(shí)限均為從建庫(kù)至2012年1月。由2名評(píng)價(jià)員按納入與排除標(biāo)準(zhǔn)獨(dú)立篩選文獻(xiàn)、提取資料和評(píng)價(jià)質(zhì)量后，采用Meta-DiSc 1.4軟件進(jìn)行合并分析。 結(jié)果最終納入12個(gè)研究。Meta分析結(jié)果顯示：SPE合并=0.79［95% CI（0.76，0.84）］、SEN合并=0.55［95% CI（0.51，0.59）］、+LR=3.2［95% CI（1.94，5.29）］、-LR=0.58［95% CI（0.45，0.74）］、DOR合并=7.56［95% CI（3.74，15.30）］、SROC曲線下面積（AUC）為0.813 1。 結(jié)論NSE對(duì)診斷惡性胸腔積液具有一定的臨床價(jià)值，可用于良惡性胸腔積液的鑒別診斷。